1. HOME
  2. Institute
  3. Departments and Groups
  4. Department of Molecular Ecology
  5. Microbial Genomics and Bioinformatics Group
  6. Staff
  7. Jörg Peplies

Dr. Jörg Peplies

Guest ScientistMicrobial Genomics and Bioinformatics Group
Address:Dr. Jörg Peplies
MPI for Marine Microbiology
Celsiusstr. 1
D-28359 Bremen
Germany
Phone:+49 421 2028 - 971
Fax:+49 421 2028 - 580

Main Research Interests

  • phylogenetic tree reconstruction and rRNA-targeted probe design
  • the ARB software package (also training for users)
  • rRNA databases and data management
  • data integration
  • bioinformatic genome and metagenome analysis
  • molecular tools for studying diversity and function of prokaryotes in
    natural (marine) environments
  • especially DNA microarrays for identification and gene expression profiling

Publications

Peer reviewed journals

Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., and Rossello-Mora R. (2008).
The All-Species Living Tree Project: a 16S rRNA-based phylogenetic tree of all sequenced type strains.
Syst. Appl. Microbiol., in press, early online doi:10.1016/j.syapm.2008.07.001

Peplies J., Kottmann R., Ludwig W., and Glöckner F. O. (2008).
A Standard Operating Procedure for Phylogenetic Inference (SOPPI) using (rRNA) marker genes.
Syst. Appl. Microbiol., in press

Richter M., Lombardot T., Kostadinov I., Kottmann R., Duhaime M. B., Peplies J., and Glöckner F. O. (2008).
JCoast - A biologist-centric software tool for data mining and comparison of prokaryotic (meta)genomes.
BMC Bioinformatics 9:177

Pruesse E., Quast C., Knittel K., Fuchs B., Ludwig W., Peplies J., and Glöckner F. O. (2007).
SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB.
Nuc. Acids Res. 35 (21): 7188-7196

Pradillon F., Schmidt A., Peplies J., and Dubilier N. (2007).
Species identification of marine invertebrate early stages by whole-larvae in situ hybridisation of 18S ribosomal RNA.
Mar. Ecol. Prog. Ser. 333: 103-116

Schübbe S., Würdemann C., Peplies J., Hayen U., Wawer C., Glöckner F.O., and Schüler D. (2006).
Transcriptional organization and regulation of magnetosome operons in Magnetospirillum gryphiswaldense.
Appl. Environ. Microbiol. 72 (9): 5757-5765

Peplies J., Lachmund C., Glöckner F.O., and Manz W. (2006).
A DNA microarray platform based on direct detection of rRNA for characterization of fresh water sediment-related prokaryotic communities.
Appl. Environ. Microbiol. 72 (7): 4829-4838

Würdemann C., Peplies J., Schübbe S., Ellrott A., Schüler D., and Glöckner F.O. (2006).
Evaluation of gene expression analysis using RNA-targeted partial genome arrays.
System. Appl. Microbiol. 29 (5): 349-357

Flies C., Peplies J., Schüler D. (2005).
A combined approach for the characterization of uncultivated magnetotactic bacteria from various aquatic environments.
Appl. Environ. Microbiol. 71 (5): 2723-2731.

Peplies J., Glöckner F.O., Amann R., and Ludwig W. (2004).
Comparative sequence analysis and oligonucleotide probe design based on 23S rRNA
genes of Alphaproteobacteria from North Sea bacterioplankton.
Syst. Appl. Microbiol., 27 (5): 573-580.

Peplies J., Lau S.C.K., Pernthaler J., Amann R., and Glöckner F.O. (2004).
Application and validation of DNA microarrays for the 16S rRNA-based analysis of marine bacterioplankton.
Environ. Microbiol., 6 (6): 638-645.

Peplies J., Glöckner F.O., and Amann, R. (2003).
Optimization strategies for DNA microarray-based detection of bacteria with 16S rRNA-targeting oligonucleotide probes.
Appl. Environ. Microbiol., 69 (3): 1397-1407.

Eilers H., Pernthaler J., Peplies J., Glöckner F.O., Gerdts G. , and Amann R. (2001).
Isolation of novel pelagic bacteria from the German Bight and their seasonal contributions to surface picoplankton.
Appl. Environ. Microbiol. 67 (11): 5134-5142.

Niemeyer C. M., Bürger W., and Peplies J. (1998).
Covalent DNA-streptavidin conjugates as building blocks for novel biometallic
nanostructures.
Angew. Chem. Int. Edit. 37 (16): 2265-2268.

Workshop Reports

Amaral-Zettler L., Peplies J., Ramette A., Fuchs B., Ludwig W., and Glöckner F.O. (2008).
Proceedings of the International Workshop on Ribosomal RNA Technology, April 7-9, 2008, Bremen, Germany.
Syst. Appl. Microbiol., in press

Book chapters

Amann R., Peplies J., and Schüler D. (2006).
Diversity and Taxonomy of Magnetotactic Bacteria.
in: "Magnetoreception and magnetosomes in bacteria (Microbiology monographs series)". 2006. Schüler, D. (editor), Springer, Heidelberg.

Current Projects

  • The SILVA rRNA databases - SSU and LSU sequences for all three domains of life
  • The All Species Living Tree Project - a 16S rRNA-based phylogenetic tree of all sequenced type strains
  • SOPPI - a standard operating procedure for phylogenetic inference using (rRNA) marker genes
  • Standards and operations for (meta)data integration - how to easily supplement your FASTA files with additional contextual data in an early stage of data processing (coming soon!)

Downloads

Peplies et al. AEM 2006

Current Employment - Alternative Address

Ribocon GmbH
Fahrenheitstr. 1
28359 Bremen
Germany

Phone: +49 421 2487053
Fax: +49 421 2208150
e-mail: jpepliesribocon.com

Ribocon is a spin-off of the MPI Bremen founded in 2005 and a Science Service Company working in the fields of Bioinformatics & Molecular Diagnostics.

Web Links