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Dr. Benedikt Geier

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Scientist

Department of Symbiosis

MPI for Marine Microbiology
Celsiusstr. 1
D-28359 Bremen
Germany

Raum: 

2246

Telefon: 

+49 421 2028-9060

Dr. Benedikt Geier
 

Hello,

I am interested in developing and applying correlative imaging approaches to disentangle the symbiotic interactions between microbes and small symbiotic animals in situ. Thereby, I focus on the integration of metabolite imaging with MALDI-MSI and imaging of the physical association of both partners using FISH and microCT.

MALDI-MSI: Matrix-assisted laser desorption/ionization mass spectrometry imaging
FISH: Fluorescence in situ hybridization for partner-specific labeling
microCT: Micro-computed X-ray tomography for high-resolution microanatomy of the host tissues

Techniques:

  • High-resolution MALDI-MSI sample preparation, imaging and integration with other microscopy techniques
  • FISH and fluorescence microscopy (CLSM, Airyscan)
  • Histology in combination with serial approaches for 3D reconstruction
  • MicroCT approaches, laboratory-based and synchrotron radiation-based (e.g. phase contrast)
  • 3D visualization including image alignment, filtering, surface reconstruction, 3D video animations and co-registration of different imaging datasets within 3D space.


Organisms:

  • Deep-sea mussels of the genus Bathymodiolus including their chemosynthetic methane- and sulfur-oxidizing symbionts
  • Common garden earthworms

Collaborations:

  • Dr. Bernhard Ruthensteiner, Bavarian State Collection of Zoology (ZSM, Munich)
  • Dr. Jörg Hammel, Helmholtz-Center Geesthacht (HZG) outstation at Deutsches Elektronen Synchrotron (DESY, Hamburg)
  • Dr. Maxim Polikarpov, European Molecular Biology Laboratory (EMBL, Hamburg Unit at the DESY)
  • Dr. Janina Oetjen, MALDI Imaging Lab, University of Bremen/Bruker Daltonics
  • Assoc. Prof. Christian Gruber and Dr. Esther Gill Mansilla, Center for Physiology and Pharmacology, Medical University of Vienna
 
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