I am interested in developing and applying correlative imaging approaches to disentangle the symbiotic interactions between microbes and small symbiotic animals in situ. Thereby, I focus on the integration of metabolite imaging with MALDI-MSI and imaging of the physical association of both partners using FISH and microCT.
MALDI-MSI: Matrix-assisted laser desorption/ionization mass spectrometry imaging
FISH: Fluorescence in situ hybridization for partner-specific labeling
microCT: Micro-computed X-ray tomography for high-resolution microanatomy of the host tissues
- High-resolution MALDI-MSI sample preparation, imaging and integration with other microscopy techniques
- FISH and fluorescence microscopy (CLSM, Airyscan)
- Histology in combination with serial approaches for 3D reconstruction
- MicroCT approaches, laboratory-based and synchrotron radiation-based (e.g. phase contrast)
- 3D visualization including image alignment, filtering, surface reconstruction, 3D video animations and co-registration of different imaging datasets within 3D space.
- Deep-sea mussels of the genus Bathymodiolus including their chemosynthetic methane- and sulfur-oxidizing symbionts
- Common garden earthworms
- Dr. Bernhard Ruthensteiner, Bavarian State Collection of Zoology (ZSM, Munich)
- Dr. Jörg Hammel, Helmholtz-Center Geesthacht (HZG) outstation at Deutsches Elektronen Synchrotron (DESY, Hamburg)
- Dr. Maxim Polikarpov, European Molecular Biology Laboratory (EMBL, Hamburg Unit at the DESY)
- Dr. Janina Oetjen, MALDI Imaging Lab, University of Bremen/Bruker Daltonics
- Assoc. Prof. Christian Gruber and Dr. Esther Gill Mansilla, Center for Physiology and Pharmacology, Medical University of Vienna