Hagen Buck-Wiese

During my PhD, I aim to analyze carbohydrates in the ocean and link their concentration and dynamics to oceanographic parameters. Oceanic cycling of carbon has a considerable influence on the climate (e.g. biological and “microbial” carbon pumps). Current knowledge of carbon cycling in the water column is based on fingerprinting of the carbon inventory. After observing a diversification of carbohydrates by microbial enzymes, I developed an extraction technique for carbohydrates from seawater. With this extraction technique, we obtained samples from the Mauritanian Upwelling System over diel cycles on a transect from shore to open ocean.

• marine carbon cycle
• molecular composition and turnover of marine dissolved organic matter
• mechanisms of carbon sequestration
• carbohydrate sequencing

• carbohydrate extraction from seawater
• enzymatic digestion
• liquid chromatography-tandem mass spectrometry

Dittmar, T., Lennatz, S., Buck-Wiese, H. et al. Enigmatic persistence of dissolved organic matter in the oceans. Nat Rev Earth Environ (accepted)

Buck-Wiese, H., Fanuel, M., Liebeke, M. et al. Discrimination of β-1,4- and β-1,3-Linkages in Native Oligosaccharides via Charge Transfer Dissociation Mass Spectrometry. J Am Soc Mass Spectrom 31 (6), 1249-1259 (2020). https://doi.org/10.1021/jasms.0c00087

Brüwer J.D. and Buck-Wiese H. (2018) Reading the book of life – omics as a universal tool across disciplines in S. Jungblut et al. (eds.), YOUMARES 8 – Oceans Across Boundaries: Learning from Each Other, Springer, https://doi.org/10.1007/978-3-319-93284-2_6

Buck-Wiese H., Burgués I., Medrano A., Fernandez-Navarrete T., Garcia M., Wieters E.A. (2018) Patterns in sexual reproduction of the dominant scleractinian corals at Rapa Nui (Easter Island): Pocillopora verrucosa and Porites lobata, Aquatic Biology, https://doi.org/10.3354/ab00691

Buck-Wiese H., Voolstra C. R., Brüwer J. D. (2017) The metaorganism frontier - incorporating microbes in an organism's response to environmental change