Diploma & Ph.D. work

Molecular techniques are currently the methods of choice to unravel the black box of microbial ecology. Within my Diploma and Ph.D. work I was using the rRNA-approach in combination with fluorescence in situ hybridization (FISH) techniques to describe the bacterial diversity and composition of limnic and marine systems like high mountain lakes, Lake Baikal, the Baltic Sea, the North Sea and the Antarctic Ocean.
I developed new protocols and 16S rRNA oligonucleotide probes for the adaptation and optimization of the FISH technique to the new habitats. My results showed, that numerous bacteria in these ecosystems might be yet uncharacterized with distinguishing marks concerning the distribution of phylogenetic groups in limnic and marine ecosystems.
Comparative phylogenetic analysis of different clone libraries from limnic systems revealed high diversity. New groups lacking any cultivated member were found and their high abundance could be confirmed by FISH with specific oligonucleotide probes.
Achromatium oxaliferum
Achromatium oxaliferum
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