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Small but versatile; key players in the marine nitrogen cycle can utilize cyanate and urea

Dec 10, 2018

The ammonia oxidizing archaea, or Thaumarchaeota, are amongst the most abundant marine microorganisms. Yet, we are still discovering which factors allow them to thrive in the ocean. A research team from the Max Planck Institute for Marine Microbiology in Bremen and the University of Vienna was now able to show that marine Thaumarchaeota have a broader metabolism than previously thought. The results are published in the journal Nature Microbiology.

The Thaumarchaeota play a key role in the marine nitrogen cycle. They gain energy for growth by converting ammonia, which is the most reduced form of inorganic nitrogen, to a more oxidized form: nitrite. These so-called ammonia oxidizing archaea were discovered little more than a decade ago, yet these organisms make up a large part of the marine microbial community, thriving in the oceans despite ammonium being present only at very low concentrations.

Even though the Thaumarchaeota are such a key part of the marine nitrogen cycle, little is known about the physiology of these small and enigmatic microorganisms. In general, they are considered to be metabolically restricted, relying on ammonia as an energy source. A new study by Katharina Kitzinger and colleagues from the Max Planck Institute for Marine Microbiology in Bremen, Germany, the University of Vienna, Austria, the Georgia Institute for Technology, USA, the Carl von Ossietzky University Oldenburg, Germany, and the University of Bremen, Germany, now reveals that this is not quite true. Rather, the authors show that marine ammonia oxidizing archaea can also utilize organic nitrogen sources.

Figure caption 1: Single-cell images of environmental marine ammonia oxidizing archaea. Panel (a) identifies the ammonia oxidizing archaea (green) and surrounding cells (blue), panel (b) reveals their uptake of cyanate. This can be determined with NanoSIMS, a technology that provides highly detailed insights into the activity of single cells. Ammonia oxidizing archaea are marked by white outlines. Scale bar is 1 μm. (Copyright: Max Planck Institute for Marine Microbiology/K. Kitzinger)  Figure caption 2: Samples for this study were taken in den Gulf of Mexico. (Copyright: Max Planck Institute for Marine Microbiology/K. Kitzinger)
Figure caption 1: Single-cell images of environmental marine ammonia oxidizing archaea. Panel (a) identifies the ammonia oxidizing archaea (green) and surrounding cells (blue), panel (b) reveals their uptake of cyanate. This can be determined with NanoSIMS, a technology that provides highly detailed insights into the activity of single cells. Ammonia oxidizing archaea are marked by white outlines. Scale bar is 1 μm. (© Max Planck Institute for Marine Microbiology/K. Kitzinger)

“We show for the first time that both environmental and cultured marine ammonia oxidizing archaea can use cyanate, a simple organic nitrogen compound, as an additional energy source“, Kitzinger explains. Further, they show these microorganisms also use that urea, another organic nitrogen compound. These findings are important as cyanate and urea are common nitrogen and energy sources in the oceans. The Thaumarchaeota’s ability to supplement their metabolism with these compounds might be one reason for their outstanding success in the oceans.   

Kitzinger is especially intrigued by how the marine ammonia oxidizing archaea are able to use cyanate. “We still aren’t sure exactly how they do it. They don't have the typical enzyme repertoire needed to use cyanate. It will be exciting to see which enzymes allow marine ammonia oxidizing archaea to use cyanate, if these organisms have an even larger metabolic versatility than we know now, and how this versatility shapes their ecology”, says Kitzinger.

Figure caption 2: Samples for this study were taken in den Gulf of Mexico. (Copyright: Max Planck Institute for Marine Microbiology/K. Kitzinger)
Figure caption 2: Samples for this study were taken in den Gulf of Mexico. (copyright: Max Planck Institute for Marine Microbiology/K. Kitzinger)

Original publication

Katharina Kitzinger, Cory C. Padilla, Hannah K. Marchant, Philipp F. Hach, Craig W. Herbold, Abiel T. Kidane, Martin Könneke, Sten Littmann, Maria Mooshammer, Jutta Niggemann, Sandra Petrov, Andreas Richter, Frank J. Stewart, Michael Wagner, Marcel M. M. Kuypers, Laura A. Bristow: Cyanate and Urea are Substrates for Nitrification by Thaumarchaeota in the Marine Environment. Nature Microbiology.

DOI: 10.1038/s41564-018-0316-2

Participating institutes

  • Max Planck Institute for Marine Microbiology, Bremen, Germany

  • Department of Microbiology and Ecosystem Science, University of Vienna, Austria

  • School of Biological Sciences, Georgia Institute of Technology, Atlanta, USA

  • Research Group for Marine Geochemistry (ICBM-MPI Bridging Group), Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University, Oldenburg, Germany

  • Marine Archaea Group, MARUM – Center for Marine Environmental Sciences, Bremen, Germany

Please direct your queries to:

Scientist

Department of Biogeochemistry

Dr. Hannah Marchant

MPI for Marine Microbiology
Celsiusstr. 1
D-28359 Bremen
Germany

Room: 

3135

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+49 421 2028-630

Dr. Hannah Marchant

PhD student

Department of Biogeochemistry

Katharina Kitzinger

MPI for Marine Microbiology
Celsiusstr. 1
D-28359 Bremen
Germany

Room: 

3134

Phone: 

+49 421 2028-646

Katharina Kitzinger

or to our press team:

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Press

Dr. Fanni Aspetsberger

MPI for Marine Microbiology
Celsiusstr. 1
D-28359 Bremen
Germany

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3248

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+49 421 2028-947

Dr. Fanni Aspetsberger
 
 
 
 
 
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