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Flow Cytometry Research Group

 

Group Leader

Group Leader

Flow Cytometry Research Group

PD Dr. Bernhard Fuchs

MPI for Marine Microbiology
Celsiusstr. 1
D-28359 Bremen
Germany

Room: 

2222

Phone: 

+49 421 2028-9350

PD Dr. Bernhard Fuchs
Flow Cytometry Group
The Flow Cytometry group in February 2021. Missing: Laura Zeugner, Taylor Priest and Peter Rücknagel. © Max Planck Institute for Marine Microbiology/A. Esken

About our Research

Flow cytometry complements the molecular biological toolbox to study microorganisms. Its capacity of fast analysis of thousands of cells per second and the simultaneous recording of multiple parameters makes flow cytometry a standard tool in plankton research. In addition, fluorescence-activatedcell sorting (FACS) enables the physical separation and enrichment of well-defined cell populations without prior cultivation. Read more about what flow cytometry is ⇒ here

In our group we continuously improve our flow cytometry methods and combine them with new molecular biological approaches to gain deeper insights into the ecological role of microbes in a range of diverse marine habitats. Find out what projects we are running ⇒ here

Since April 2018 we are a Research Group within the Department of Molecular Ecology.

Our approach
© Max Planck Institute for Marine Microbiology/A. Grieb

In this cartoon we summarized our approach, how we combine several methods for the specific enrichment of microbes by flow cytometric sorting: (1) Using Fluorescence in situ Hybridization (FISH), we fluorescently label specific microbial species in our water sample (e.g. from Helgoland Roads). (2) Based on their fluorescence signal, depicted here in green, cells are sorted by flow cytometry and collected in a vial. (3) The DNA of the sorted cells is sequenced and further analyzed. Alternatively, also the proteins of the sorted cells can be determined.

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